Introduction
- Scientists at the Indian Institute of Science (IISc.) in Bengaluru have successfully created a synthetic human antibody capable of neutralizing potent neurotoxins found in the venom of highly toxic snakes.
Synthetic Antibody against Snake Venom
- Approach: The team utilized a method previously employed to screen antibodies against HIV and COVID-19 to synthesize the new venom-neutralizing antibody.
- Targeted Region: The developed antibody targets a conserved region within the core of a major toxin called the three-finger toxin (3FTx) present in elapid venom.
- Library of Antibodies: The team designed a library of artificial antibodies from humans displayed on yeast cell surfaces and screened them for binding to 3FTxs from different elapid snakes worldwide.
- Effective Binding: After rigorous screening, one antibody emerged capable of binding strongly to various 3FTxs, displaying effectiveness across different elapid species.
Challenges with Current Anti-venom
- Animal-Based Production: Existing anti-venom production involves injecting snake venom into equines and collecting antibodies from their blood, leading to therapeutically redundant antibodies due to exposure to various microorganisms.
- Efficacy Concerns: Research indicates that less than 10% of anti-venom contains antibodies specifically targeting snake venom toxins, raising concerns about efficacy.
Animal Model Testing
- Efficacy in Mice: Mice injected with a toxic 3FTx along with the antibody survived past the 24-hour observation window, while those given only the toxin succumbed within four hours.
- Versatility: The antibody showed effectiveness against the venom of different elapid species, including the monocled cobra and black mamba, with nearly 15 times the potency of conventional products.
- Delayed Administration: Crucially, administering the antibody after a time delay still successfully saved the mice, highlighting its potential for delayed treatment.
